Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
WDR5

Cell type

Cell type Class
Neural
Cell type
IMR-32
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
WDR5_ChIPseq
cell line
IMR32 cells
cell type
Neuroblastoma
treatment
siCtrl
chip antibody
WDR5 (Bethyl, A302-429A)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6346079
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
53539084
Reads aligned (%)
95.9
Duplicates removed (%)
23.2
Number of peaks
22709 (qval < 1E-05)

hg19

Number of total reads
53539084
Reads aligned (%)
95.5
Duplicates removed (%)
24.0
Number of peaks
22513 (qval < 1E-05)

Base call quality data from DBCLS SRA